Paper Chromatography Terms

Paper Chromatography Terms-36
For protection from ingestion, inhalation and irradiation of the radioactive product, these processes were performed in a box made of Plexiglas.

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The definition of chromatography is a process of separating out different parts of chemical mixtures onto an absorbent material that can then be individually analyzed because different parts are caught on the material at different rates.

Any of various techniques for the separation of complex mixtures that rely on the differential affinities of substances for a mobile (gas or liquid) medium and for a stationary adsorbing (liquid or solid) medium through which they pass, such as paper, gelatin, or silica.

These impurities arise from incomplete labeling, breakdown of the labeled products over time caused by instability, and introduction of extraneous labeled ingredients during synthesis.

Impurities can cause altered in vivo bio distribution after administration which can result in an unnecessary radiation dose to the patient. 3 chromatography paper sheets were cut into 1 cm × 8 cm strips and placed into empty 10 ml glass pharmaceutical vials.

For these reasons, the US Pharmacopeia and US Food and Drug Administration have set limits on impurities in different radiopharmaceuticals that must not be exceeded in clinical operations. Approximately 1 ml of the appropriate solvent was placed into the vial and two droplets of radioactive solution were dropped onto it, forming a spot about 1 cm in diameter.

Radiochemical impurities were checked using the two-solvent system: (a) saline solution (Na Cl 0.9%) as the mobile phase on Whatman no. After drying the droplets, the strips were placed in the appropriate solvent and the solvent was allowed to migrate until it reached the top of the strip. After the strip had dried, it was cut into eight 1 cm pieces and the radioactivity of each segment was measured in an alpha and beta counter (LB123 UMO Berthold) for 1 min. The chromatography spectra are shown in Figures 1 and 2 and indicate low levels of impurities for these microspheres.

Radiochemical purity Paper chromatography was used to determine the radiochemical impurities in the radioactive microspheres.

The radiochemical purity of a radiopharmaceutical is defined as the fraction of total activity in the desired chemical form in the sample [18-20].

The term has been extended to include other methods utilizing the same principle, although no colors are produced in the column. The mobile phase of chromatography refers to the fluid that carries the mixture of substances in the sample through the adsorptive material.

The stationary or adsorbent phase refers to the solid material that takes up the particles of the substance passing through it.


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